Cystic fibrosis serum alpha-l-fucosidase: confirmation of normal activity levels and normal kinetic and isoelectric focusing properties.
Publication/Presentation Date
7-17-1980
Abstract
Comparable alpha-L-fucosidase activity was found for normal and cystic fibrosis sera, 7.8 +/- 3.3 and 9.8 +/- 4.7 nmol/min/ml, respectively. Isoelectric focusing of normal and cystic fibrosis sera alpha-L-fucosidase revealed similar isoelectric profiles with most activity between isoelectric points of 4.7--5.4. Kinetic analysis of normal and cystic fibrosis sera alpha-L-fucosidase revealed identical apparent Michaelis constants for the 4-methylumbelliferyl substrate (51 +/- 6 mumol/l and 50 +/- 8 mumol/l, respectively), similar pH optima curves (both have broad optima between pH 4.8 and 6.5) and identical thermostability curves at three different preincubation temperatures (37 degrees, 45 degrees and 55 degrees C).
Volume
105
Issue
1
First Page
131
Last Page
135
ISSN
0009-8981
Published In/Presented At
Alhadeff, J. A., & Watkins, P. (1980). Cystic fibrosis serum alpha-l-fucosidase: confirmation of normal activity levels and normal kinetic and isoelectric focusing properties. Clinica chimica acta; international journal of clinical chemistry, 105(1), 131–135. https://doi.org/10.1016/0009-8981(80)90102-3
Disciplines
Medicine and Health Sciences
PubMedID
7398081
Department(s)
Department of Medicine
Document Type
Article