Gel filtration of sialoglycoproteins.
Publication/Presentation Date
7-1-1978
Abstract
The role of sialic acid in the gel-filtration behaviour of sialoglycoproteins was investigated by using the separated isoenzymes of purified human liver alpha-L-fucosidase and several other well-known sialic acid-containing glycoproteins (fetuin, alpha1-acid glycoprotein, thyroglobulin and bovine submaxillary mucin). For each glycoprotein studied, gel filtration of its desialylated derivative gave an apparent molecular weights much less than that expected just from removal of sialic acid. For the lower-molecular-weight glycoproteins (fetuin and alpha1-acid glyocprotein), gel filtration of the sialylated molecules led to apparent molecular weights much larger than the known values. The data indicate that gel filtration cannot be used for accurately determining the molecular weights of at least some sialoglycoproteins.
Volume
173
Issue
1
First Page
315
Last Page
319
ISSN
0264-6021
Published In/Presented At
Alhadeff J. A. (1978). Gel filtration of sialoglycoproteins. The Biochemical journal, 173(1), 315–319. https://doi.org/10.1042/bj1730315
Disciplines
Medicine and Health Sciences
PubMedID
356853
Department(s)
Department of Medicine
Document Type
Article