Radioimmunochemical evidence for a role of carbohydrate in antigenic determinant(s) on human liver alpha-L-fucosidase.
Publication/Presentation Date
10-15-1984
Abstract
A competitive-binding radioimmunoassay method was employed to investigate the role of carbohydrate in antigenic determinant(s) of human liver alpha-L-fucosidase. Competition curves were used to quantify the concentrations of competitors needed to cause 30% inhibition of the precipitation of 125I-labelled alpha-L-fucosidase. The isoelectric forms of alpha-L-fucosidase, which are related by sialic acid residues, were separated preparatively and used as competitors in the radioimmunoassay. A pattern of increasing effectiveness as competitors with increasing acidity of the forms was found, suggesting that sialic acid may be involved in the antigenic determinant(s) of alpha-L-fucosidase. Specificity was exhibited when sugar and sugar derivatives were used as competitors in the radioimmunoassay: a 51-fold range of competitive ability was found, and sialic acids (N-acetylneuraminic acid and N-glycollylneuraminic acid) and colominic acid (a polymer of N-acetylneuraminic acid) were the best competitors. The results of our studies suggest that carbohydrate contributes to antigenic determinant(s) of alpha-L-fucosidase and that sialic acid is probably the major sugar involved.
Volume
223
Issue
2
First Page
293
Last Page
298
ISSN
0264-6021
Published In/Presented At
Alhadeff, J. A., & Andrews-Smith, G. L. (1984). Radioimmunochemical evidence for a role of carbohydrate in antigenic determinant(s) on human liver alpha-L-fucosidase. The Biochemical journal, 223(2), 293–298. https://doi.org/10.1042/bj2230293
Disciplines
Medicine and Health Sciences
PubMedID
6208895
Department(s)
Department of Medicine
Document Type
Article