High-performance liquid chromatographic analysis of fucoganglioside hydrolysis by alpha-L-fucosidase.
Publication/Presentation Date
9-1-1990
Abstract
A sensitive HPLC method has been developed for monitoring fucoganglioside hydrolysis by purified alpha-L-fucosidase. The high-resolution method employs a Lichrosorb-NH2 column, a 10-min isocratic elution with potassium phosphate/acetonitrile buffer, detection of ganglioside products with a uv monitor at 195 nm, and quantification of low picomolar amounts of these gangliosides with an integrator. The usefulness of the HPLC method has been exemplified by using it to demonstrate the hydrolysis of gangliosides fucosyl-GM1 and fucosyl-GD1b by purified human liver alpha-L-fucosidase in the absence of activator proteins and/or detergents.
Volume
189
Issue
2
First Page
209
Last Page
212
ISSN
0003-2697
Published In/Presented At
Johnson, S. W., Masserini, M., & Alhadeff, J. A. (1990). High-performance liquid chromatographic analysis of fucoganglioside hydrolysis by alpha-L-fucosidase. Analytical biochemistry, 189(2), 209–212. https://doi.org/10.1016/0003-2697(90)90109-m
Disciplines
Medicine and Health Sciences
PubMedID
2281865
Department(s)
Department of Medicine
Document Type
Article