S-antigen. Identification of human T-cell lymphocyte proliferation sites.
Publication/Presentation Date
10-1-1990
Abstract
Immune responses to normal retinal proteins, including S-antigen, have been demonstrated in patients with a variety of retinal disorders, as well as in those who have received panretinal laser photocoagulation. T-cell lymphocytes (T cells) have been implicated in the pathogenesis of several ocular inflammatory diseases of possible autoimmune etiology. We used synthetic peptides that correspond to the amino acid sequence of S-antigen in lymphocyte proliferation assays to identify specific sites in the molecule recognized by human T cells. Ten patients with type II diabetes were studied before and after initial panretinal laser photocoagulation for proliferative diabetic retinopathy. T-cell responses, expressed as a stimulation index, to S-antigen and peptides were negative in all patients before treatment. Three weeks after panretinal laser photocoagulation, eight of 10 assays were positive (stimulation index greater than 2; P less than .01) when lymphocytes were stimulated with peptide BSA(273-292); six of nine were positive (P less than .01) with peptide BSA(303-332); and six of six were positive (P less than .001) with peptide BSA(343-362). Our study identifies several specific sites in S-antigen that elicit human immune responses. The implications of these findings with regard to the pathogenesis and treatment of autoimmune uveitis are discussed.
Volume
108
Issue
10
First Page
1470
Last Page
1473
ISSN
0003-9950
Published In/Presented At
Vrabec, T. R., Reber, R. N., Magargal, L. E., & Donoso, L. A. (1990). S-antigen. Identification of human T-cell lymphocyte proliferation sites. Archives of ophthalmology (Chicago, Ill. : 1960), 108(10), 1470–1473. https://doi.org/10.1001/archopht.1990.01070120118040
Disciplines
Medicine and Health Sciences
PubMedID
2222280
Department(s)
Department of Medicine
Document Type
Article