Adrm1 interacts with Atp6v0d2 and regulates osteoclast differentiation.
Publication/Presentation Date
12-18-2009
Abstract
Bone homeostasis is tightly regulated by matrix-producing osteoblasts and bone-resorbing osteoclasts. During osteoclast development, mononuclear preosteoclasts derived from myeloid cells fuse together to form multinucleated, giant cells. Previously, we reported that the d2 isoform of the vacuolar (H(+)) ATPase V0 domain (Atp6v0d2) plays an important role in osteoclast maturation and bone formation. To understand how Atp6v0d2 controls osteoclast maturation, we have performed a yeast two-hybrid screen using full-length Atp6v0d2 as the bait, and identified adhesion-regulating molecule 1 protein (Adrm1) as a potential functional partner of Atp6v0d2. The interaction between Atp6v0d2 and Adrm1 was confirmed in yeast and invivo using immunoprecipitation assays. We also show that Adrm1 is required for cell migration and osteoclast maturation.
Volume
390
Issue
3
First Page
585
Last Page
590
ISSN
1090-2104
Published In/Presented At
Kim, T., Ha, H. I., Kim, N., Yi, O., Lee, S. H., & Choi, Y. (2009). Adrm1 interacts with Atp6v0d2 and regulates osteoclast differentiation. Biochemical and biophysical research communications, 390(3), 585–590. https://doi.org/10.1016/j.bbrc.2009.10.010
Disciplines
Medicine and Health Sciences
PubMedID
19818731
Department(s)
Department of Medicine
Document Type
Article