Ion exchange activity in pulmonary artery smooth muscle cells: the response to hypoxia.
Publication/Presentation Date
2-1-2001
Abstract
The purposes of this study were to determine 1) the presence of the major ion transport activities that regulate cytoplasmic pH (pH(c)) in cat pulmonary artery smooth muscle cells, i.e., Na+/H+ and the Na+-dependent and -independent Cl-/HCO3- exchange, 2) whether pH(c) changes in cells from small (SPAs) and large (LPAs) pulmonary arteries during hypoxia, and 3) whether changes in pH(c) are due to changes in the balance of exchange activities. Exchange activities as defined by physiological maneuvers rather than molecular identity were ascertained with fluorescence microscopy to document changes in the ratio of the pH(c) indicator 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein. Steady-state pH(c) was higher in LPA than in SPA normoxic smooth muscle cells. SPAs and LPAs possessed all three transport activities; in HCO3- containing normoxic solutions, Cl-/HCO3- exchange rather than Na+/H+ exchange set the level of pH(c); in HCO3- containing hypoxic solutions, pH(c) increased in SPA and decreased in LPA cells; altering the baseline pH(c) of a cell type to that of the other did not change the direction of the pH(c) response during hypoxia. The absence of Na+ prevented hypoxia-induced alkalinization in SPA cells; in both cell types, inhibiting the Cl-/HCO3- exchange activities reversed the normal direction of pH(c) changes during hypoxia.
Volume
280
Issue
2
First Page
264
Last Page
271
ISSN
1040-0605
Published In/Presented At
Madden, J. A., Ray, D. E., Keller, P. A., & Kleinman, J. G. (2001). Ion exchange activity in pulmonary artery smooth muscle cells: the response to hypoxia. American journal of physiology. Lung cellular and molecular physiology, 280(2), L264–L271. https://doi.org/10.1152/ajplung.2001.280.2.L264
Disciplines
Medicine and Health Sciences
PubMedID
11159005
Department(s)
Department of Medicine
Document Type
Article