Technetium-99m-labeled proteins for imaging inflammatory foci.
Publication/Presentation Date
1-1-1991
Abstract
Polyclonal human IgG (IgG), antinuclear antibody (TNT-1), and human serum albumin (HSA), were labeled with 99mTc by a method recently developed in our laboratory, and administered i.v., each to a separate group of five mice, bearing inflammatory foci induced by an i.m. injection of 40 microL turpentine or 5 x 10(8) E. coli and 5 x 10(8) Entercocci. TNT-1 labeled with 125I served as a control and 67Ga-citrate as a "gold standard". At 4 or 24 h post injection, animals were imaged and sacrificed for tissue distribution studies. At 4 h in the turpentine group, the abscess-to-muscle ratios were: 67Ga, 4.8 +/- 2.1, 125I-TNT-1, 4.3 +/- 1; 99mTc-TNT-1, 3.5 +/- 1.8; 99mTc-IgG, 3.9 +/- 0.6; and 99mTc-HSA, 4.3 +/- 1. In the microorganism group, these ratios were 2.6 +/- 0.6, 3.3 +/- 0.5, 3.4 +/- 0.08, 3 +/- 1.1 and 4.1 +/- 0.6, respectively. Autoradiographic examination of infected tissues indicated that leakage of labeled proteins into interstitial space due to increased capillary permeability may be one of the major mechanisms of uptake.
Volume
18
Issue
6
First Page
605
Last Page
612
ISSN
0883-2897
Published In/Presented At
Thakur, M. L., DeFulvio, J., Park, C. H., Damjanov, A., Yaghsezian, H., Jungkind, D., Epstein, A., & McAfee, J. G. (1991). Technetium-99m-labeled proteins for imaging inflammatory foci. International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology, 18(6), 605–612. https://doi.org/10.1016/0883-2897(91)90030-o
Disciplines
Medicine and Health Sciences
PubMedID
1743984
Department(s)
Department of Obstetrics and Gynecology
Document Type
Article