Variation in antisperm antibody results using different assays.
Publication/Presentation Date
1-1-1995
Abstract
PROBLEM: The detection of various types of antisperm antibodies (ASA) in the serum varies among different assays. This variation may influence the diagnosis and management of infertile couples who are tested for such immunologic factors. This prospective study was conducted to determine the variation in the results of ASA as measured by the sperm immobilization (SI), sperm agglutination (SA), and the indirect immunobead (IB) assays.
METHOD: The sera of 79 patients that tested positive for ASA by at least one of the assays listed above were concurrently tested with all three assays.
RESULTS: Using an individual ASA assay, 66 (84%), 26 (33%) or 36 (46%) of sera tested positive by the SA, SI, or IB assays, respectively. However, using a combination of assays, 67 (85%), 78 (99%) or 40 (51%) of sera tested positive using either the SA+SI, SA+IB or SI+IB assays, respectively.
CONCLUSION: These findings suggest that the utilization of different assays to detect ASA may detect sera that are positive for ASA with more reliability than single assay testing.
Volume
33
Issue
1
First Page
140
Last Page
143
ISSN
1046-7408
Published In/Presented At
Peters, A. J., Ivanovic, M., & Jeyendran, R. S. (1995). Variation in antisperm antibody results using different assays. American journal of reproductive immunology (New York, N.Y. : 1989), 33(1), 140–143. https://doi.org/10.1111/j.1600-0897.1995.tb01151.x
Disciplines
Medicine and Health Sciences
PubMedID
7619230
Department(s)
Department of Obstetrics and Gynecology
Document Type
Article