Chemotaxis of macrophages by a peritoneal fluid protein in women with endometriosis.

Publication/Presentation Date

5-1-1997

Abstract

OBJECTIVE: To expand on a preliminary study comparing the chemotactic potential of peritoneal fluid (PF) from women with and without endometriosis and to characterize this activity via immunosuppressants and a protease.

DESIGN: Case control study.

SETTING: University center.

PATIENT(S): Fifty-nine women with endometriosis and 44 without, undergoing laparoscopy.

INTERVENTION(S): Collection of PF, endometriotic, ovarian, and endometrial biopsies at laparoscopy.

MAIN OUTCOME MEASURE(S): Chemotactic activity of PF was tested via an in vitro assay alone and in the presence of immunosuppressants cyclosporin A (CSA), FK506, rapamycin, and type XVII-b(S-V8) protease and in media incubated with endometriotic, ovarian, or endometrial biopsy specimens.

RESULT(S): The PF from women with endometriosis had significantly greater chemotactic activity (cells per well, mean +/- SD) than without endometriosis (142 +/- 39 versus 48 +/- 17). Cyclosporin A significantly inhibited the chemotactic activity of the endometriotic PF; FK506 and rapamycin did not. Incubation of media with endometriotic tissue, but not ovarian or endometrial, for > or = 7 hours displayed chemotactic activity. Protease type XVII-b(S-V8) added to endometriotic PF inhibited this chemotactic activity.

CONCLUSION(S): Peritoneal fluid from patients with endometriosis contains a protein chemotactic factor attracting inflammatory cells into the peritoneal cavity, possibly secreted by endometriotic implants. This chemotactic factor may be a member of the immunophilin family because of its inhibition profile.

Volume

67

Issue

5

First Page

865

Last Page

869

ISSN

0015-0282

Disciplines

Medicine and Health Sciences

PubMedID

9130891

Department(s)

Department of Obstetrics and Gynecology

Document Type

Article

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