T lymphocyte responses to antigens of gram-negative bacteria in pyelonephritis.
Publication/Presentation Date
10-1-1993
Abstract
We showed previously that large numbers of T lymphocytes accumulate within a few days in the kidneys of rats with ascending pyelonephritis induced with Escherichia coli or Pseudomonas aeruginosa. CD4+ T cells propagated from the lesions exhibited MHC-restricted proliferative responses to formalin-fixed bacteria of the species used to induce infection. In the present study we investigated further the nature of the antigens responsible for the T cell proliferation and studied the ability of different bacterial strains and species to produce proliferative responses. We found that heat-killed bacteria were more stimulatory than formalin-fixed bacteria, and that soluble supernatants of heat-killed organism were also effective. The stimulatory effects of supernatants were destroyed by trypsin and the responses were MHC-restricted. Twelve different E. coli strains, with or without characteristics of uropathogenicity in humans, were all highly stimulatory to T cells derived from a kidney infected with a single E. coli strain. Strains of Klebsiella pneumoniae, Enterobacter aerogenes, and Serratia marcescens--species of Enterobacteriaceae closely related to E. coli--were also stimulatory, whereas more distantly related bacteria--Proteus, Morganella, and P. aeruginosa--were not. T cells propagated from kidneys infected with P. aeruginosa responded to supernatants of this organism, but not to E. coli supernatants. We conclude that a protein antigen (or antigens) shared by strains of E. coli and related Enterobacteriaceae, but not by other gram-negative bacteria, produce MHC-restricted proliferative responses of CD4+ T cells that infiltrate rat kidneys infected with E. coli.
Volume
69
Issue
1
First Page
36
Last Page
42
ISSN
0090-1229
Published In/Presented At
Wilz, S. W., Kurnick, J. T., Pandolfi, F., Rubin, R. H., Warren, H. S., Goldstein, R., Kersten, C. M., & McCluskey, R. T. (1993). T lymphocyte responses to antigens of gram-negative bacteria in pyelonephritis. Clinical immunology and immunopathology, 69(1), 36–42. https://doi.org/10.1006/clin.1993.1147
Disciplines
Medicine and Health Sciences
PubMedID
8403542
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article