Sequence and genomic organization of the human G-protein Golfalpha gene (GNAL) on chromosome 18p11, a susceptibility region for bipolar disorder and schizophrenia.
Publication/Presentation Date
9-1-2000
Abstract
The sequence and genomic organization of the human Golfalpha (GNAL) gene were determined. The human GNAL gene was found to contain 12 coding exons, and it spans over 80 kb on chromosome 18p11. 5' RACE analysis suggested an additional transcription initiation start site. Sequence analysis of the putative promoter region revealed conserved binding sites for several transcription factors. Sequence analysis of the 3'-untranslated region revealed the presence of two Alu sequences and two polyadenylation signals. 3' RACE analysis confirmed the functionality of the most downstream poly-a signal. The human GNAL was found to be expressed as a single transcript of about 5.9 kb in the brain. One highly informative dinucleotide repeat was found in intron 5. Additionally, a processed pseudogene for asparagine synthetase was found about 6 kb upstream of the GNAL gene. Knowledge of the sequence and structure of the human GNAL gene provides essential information for further analysis of the GNAL locus at chromosome 18p11 which has been linked to bipolar disorder and schizophrenia.
Volume
5
Issue
5
First Page
495
Last Page
501
ISSN
1359-4184
Published In/Presented At
Vuoristo, J. T., Berrettini, W. H., Overhauser, J., Prockop, D. J., Ferraro, T. N., & Ala-Kokko, L. (2000). Sequence and genomic organization of the human G-protein Golfalpha gene (GNAL) on chromosome 18p11, a susceptibility region for bipolar disorder and schizophrenia. Molecular psychiatry, 5(5), 495–501. https://doi.org/10.1038/sj.mp.4000758
Disciplines
Medicine and Health Sciences
PubMedID
11032382
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article