Sample-to-answer direct real-time PCR detection of
Publication/Presentation Date
7-9-2024
Abstract
UNLABELLED: Emerging tick-borne illnesses, such as anaplasmosis, babesiosis, or ehrlichiosis, are caused by obligate intracellular pathogens that have clinically comparable presentations. Diagnostics used in laboratories today are serologic assays and blood smear analyses, which have known diagnostic limits. This study evaluated the performance of a sample-to-answer direct real-time PCR laboratory-developed test for the multiplex qualitative detection of
IMPORTANCE: This work demonstrates that detection of tick-borne illnesses, such as anaplasmosis, babesiosis, or ehrlichiosis, can be performed directly from whole blood with no extraction. The assay described here has a high positive and negative percent agreement with existing methods and is used as the standard of care. An increasing incidence of tick-borne illness combined with shortage of well-trained technologists to perform traditional manual testing, testing options that can be adapted to various lab settings, are of the utmost importance.
First Page
0065524
Last Page
0065524
ISSN
2165-0497
Published In/Presented At
Colasante, G., Makari, K., Hummel, T. I., & Murphy, C. (2024). Sample-to-answer direct real-time PCR detection of Anaplasma phagocytophilum, Ehrlichia spp., and Babesia spp. infections in whole-blood specimens. Microbiology spectrum, e0065524. Advance online publication. https://doi.org/10.1128/spectrum.00655-24
Disciplines
Medicine and Health Sciences
PubMedID
38980020
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article