Routine forensic use of the mitochondrial 12S ribosomal RNA gene for species identification.
Publication/Presentation Date
11-1-2007
Abstract
Since July 2004, Mitotyping Technologies has been amplifying and sequencing a approximately 150 base pair fragment of mitochondrial DNA (mtDNA) that codes for 12S ribosomal RNA, to identify the species origin of nonhuman casework samples. The approximately 100 base pair sequence product is searched at http://www.ncbi.nlm.nih.gov/BLAST and the species match is reported. The use of this assay has halved the number of samples for which no mtDNA results are obtained and is especially useful on hairs and degraded samples. The availability of species determination may aid forensic investigators in opening or closing off lines of inquiry where a highly probative but challenging sample has been collected.
Volume
52
Issue
6
First Page
1305
Last Page
1307
ISSN
0022-1198
Published In/Presented At
Melton, T., & Holland, C. (2007). Routine forensic use of the mitochondrial 12S ribosomal RNA gene for species identification. Journal of forensic sciences, 52(6), 1305–1307. https://doi.org/10.1111/j.1556-4029.2007.00553.x
Disciplines
Medicine and Health Sciences
PubMedID
17868265
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article