Clinical experience with flow cytometric separation of human X- and Y-chromosome bearing sperm.
Publication/Presentation Date
12-1-1999
Abstract
Numerous methods to separate human X- and Y-bearing sperm have been reported with unconfirmed separation after DNA analysis and inconsistent birth results. Successful flow cytometric separation of sperm resulting in alteration of the sex ratio of young born has been demonstrated in several animal species. Flow cytometric separation of human X- and Y-bearing sperm (MicroSort) has been confirmed after DNA analysis by fluorescence in situ hybridization (FISH). Pregnancies and births have resulted from the use of MicroSort after intrauterine insemination (IUI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).
Volume
52
Issue
8
First Page
1435
Last Page
1440
ISSN
0093-691X
Published In/Presented At
Fugger E. F. (1999). Clinical experience with flow cytometric separation of human X- and Y-chromosome bearing sperm. Theriogenology, 52(8), 1435–1440. https://doi.org/10.1016/s0093-691x(99)00228-9
Disciplines
Medicine and Health Sciences
PubMedID
10735087
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article