Androgen receptor regulates expression of skeletal muscle-specific proteins and muscle cell types.

Publication/Presentation Date

10-1-2004

Abstract

C2C12 myoblasts expressing the androgen receptor (AR) were used to analyze the role of androgen-AR signaling pathway in skeletal muscle development. Marked up-regulation of AR expression was observed in differentiated myotubes. A nuclear run-on transcription assay demonstrated that transcription of the AR gene is increased during skeletal muscle cell differentiation. Regulation of skeletal muscle-specific protein expression by the androgen-AR signaling pathway was further analyzed using quadriceps skeletal muscle from wild-type (WT) and AR knock-out (ARKO) male mice. A histological analysis of quadriceps skeletal muscle indicates no morphological differences between ARKO and WT mice. However, the androgen-AR signaling pathway increases expression of slow-twitch-specific skeletal muscle proteins and downregulates fast-twitch-specific skeletal muscle proteins, resulting in an increase of slow-twitch muscle fiber type cells in quadriceps muscle.

Volume

25

Issue

1

First Page

27

Last Page

32

ISSN

1355-008X

Disciplines

Medicine and Health Sciences

PubMedID

15545703

Department(s)

Department of Pathology and Laboratory Medicine

Document Type

Article

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