Quantitative reverse transcription polymerase chain reaction-based detection of thyroid-specific gene expression in fine-needle aspirate for thyroid cancer recurrence evaluation: a case report and review of the literature.
Publication/Presentation Date
11-1-2015
Abstract
BACKGROUND: Despite improved surveillance for patients after total thyroidectomy for cancer, there has yet to be a diagnostic method that detects recurrence with 100% accuracy.
METHODS: A 60-year-old woman with a family history of papillary thyroid cancer (PTC) underwent total thyroidectomy and radioactive iodine ablation. On postoperative surveillance, ultrasound examination of the neck demonstrated a focus concerning for recurrence and a fine-needle aspirate (FNA) was performed. The cytology report was nondiagnostic and, hence, RNA was extracted from the specimen followed by reverse transcription (cDNA), and quantitative real-time polymerase chain reaction (qRT-PCR) to detect thyroid-specific gene expression (thyroglobulin =Tg; sodium-iodide symporter = NIS; thyroperoxidase = TPO).
RESULTS: Expression of select thyroid-specific genes was demonstrated, and given the patient's remarkable cancer and family history, surgical resection was elected. Final pathology demonstrated follicular adenoma.
CONCLUSION: This case demonstrates a novel approach used in the evaluation for recurrent thyroid cancer as an adjunct to FNA cytology.
Volume
37
Issue
11
First Page
165
Last Page
168
ISSN
1097-0347
Published In/Presented At
Azoury, S. C., Aufforth, R., He, M., Yang, Z., Nilubol, N., & Kebebew, E. (2015). Quantitative reverse transcription polymerase chain reaction-based detection of thyroid-specific gene expression in fine-needle aspirate for thyroid cancer recurrence evaluation: a case report and review of the literature. Head & neck, 37(11), E165–E168. https://doi.org/10.1002/hed.24054
Disciplines
Medicine and Health Sciences
PubMedID
25784309
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article