Clonally expanded CD4+ T cells can produce infectious HIV-1 in vivo.
Publication/Presentation Date
2-16-2016
Abstract
Reservoirs of infectious HIV-1 persist despite years of combination antiretroviral therapy and make curing HIV-1 infections a major challenge. Most of the proviral DNA resides in CD4(+)T cells. Some of these CD4(+)T cells are clonally expanded; most of the proviruses are defective. It is not known if any of the clonally expanded cells carry replication-competent proviruses. We report that a highly expanded CD4(+) T-cell clone contains an intact provirus. The highly expanded clone produced infectious virus that was detected as persistent plasma viremia during cART in an HIV-1-infected patient who had squamous cell cancer. Cells containing the intact provirus were widely distributed and significantly enriched in cancer metastases. These results show that clonally expanded CD4(+)T cells can be a reservoir of infectious HIV-1.
Volume
113
Issue
7
First Page
1883
Last Page
1888
ISSN
1091-6490
Published In/Presented At
Simonetti, F. R., Sobolewski, M. D., Fyne, E., Shao, W., Spindler, J., Hattori, J., Anderson, E. M., Watters, S. A., Hill, S., Wu, X., Wells, D., Su, L., Luke, B. T., Halvas, E. K., Besson, G., Penrose, K. J., Yang, Z., Kwan, R. W., Van Waes, C., Uldrick, T., … Maldarelli, F. (2016). Clonally expanded CD4+ T cells can produce infectious HIV-1 in vivo. Proceedings of the National Academy of Sciences of the United States of America, 113(7), 1883–1888. https://doi.org/10.1073/pnas.1522675113
Disciplines
Medicine and Health Sciences
PubMedID
26858442
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article