Improved biuret procedure for routine determination of urinary total proteins in clinical proteinuria.
Publication/Presentation Date
3-1-1975
Abstract
This communication describes and evaluates an improved routine methodology for quantitating clinical proteinuria. Based on investigations of Piscator and of Savory et al., a modified Tsuchiya's reagent (ethanolic HCI-phosphotungstic acid) is used to precipitate proteins at 56 degrees C, followed by biuret spectrophotometry at 540 nm. The accuracy of the proposed procedure was assessed by comparisons with results obtained by using an ultrafiltration membrane that retains solutes with an average molecular weight in excess of 10 000 for separating of urinary proteins before they are measured with the biuret reaction. Precision of the method (coefficient of variation) is typically 2-3%.
Volume
21
Issue
3
First Page
398
Last Page
401
ISSN
0009-9147
Published In/Presented At
Rice E. W. (1975). Improved biuret procedure for routine determination of urinary total proteins in clinical proteinuria. Clinical chemistry, 21(3), 398–401.
Disciplines
Medicine and Health Sciences
PubMedID
1112050
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article