A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker.
Publication/Presentation Date
12-19-2006
Abstract
Two isoforms of proliferating cell nuclear antigen (PCNA) have been observed in breast cancer cells. Commercially available antibodies to PCNA recognize both isoforms and, therefore, cannot differentiate between the PCNA isoforms in malignant and nonmalignant breast epithelial cells and tissues. We have developed a unique antibody that specifically detects a PCNA isoform (caPCNA) associated with breast cancer epithelial cells grown in culture and breast-tumor tissues. Immunostaining studies using this antibody suggest that the caPCNA isoform may be useful as a marker of breast cancer and that the caPCNA-specific antibody could potentially serve as a highly effective detector of malignancy. We also report here that the caPCNA isoform functions in breast cancer-cell DNA replication and interacts with DNA polymerase delta. Our studies indicate that the caPCNA isoform may be a previously uncharacterized detector of breast cancer.
Volume
103
Issue
51
First Page
19472
Last Page
19477
ISSN
0027-8424
Published In/Presented At
Malkas, L. H., Herbert, B. S., Abdel-Aziz, W., Dobrolecki, L. E., Liu, Y., Agarwal, B., Hoelz, D., Badve, S., Schnaper, L., Arnold, R. J., Mechref, Y., Novotny, M. V., Loehrer, P., Goulet, R. J., & Hickey, R. J. (2006). A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker. Proceedings of the National Academy of Sciences of the United States of America, 103(51), 19472–19477. https://doi.org/10.1073/pnas.0604614103
Disciplines
Medicine and Health Sciences
PubMedID
17159154
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article