Application of Molecular Cytogenetic Techniques in a Case Study of Human Cutaneous Metastatic Melanoma.
Publication/Presentation Date
12-2001
Abstract
Consistent structural chromosome rearrangements have rarely been identified in adult solid tumors. The introduction of advanced molecular cytogenetic techniques has provided new ways of analyzing highly complex karyotypes commonly encountered in these malignancies. This study describes a detailed molecular cytogenetic analysis of a sporadic human cutaneous melanoma biopsy, M92-047, using a combination of G-banding, fluorescence in situ hybridization (FISH), chromosome microdissection, and comparative genomic hybridization (CGH). G-banding revealed that this tumor was composed primarily of closely related near-diploid and near-tetraploid cell subpopulations containing several clonal numerical and structural chromosome alterations. Fluorescence in situ hybridization using whole chromosome painting probes and chromosome arm painting probes, was employed to verify the rearranged chromosomes; dic(1;4), der(8)t(1;8), and der(15)t(6;15), whereas marker chromosomes dic(8;1;16), der(12)t(9;12), and der(17)t(13;17) were discerned by chromosome microdissection and subsequent reverse in situ hybridization (rev ish) analysis. Comparative genomic hybridization illustrated DNA copy number changes in good agreement with the karyotypic analysis. Although this line exhibits recurrent alterations representative of melanoma, two unique breakpoints--1p13 and 8p21--were identified in two different rearranged chromosomes, suggesting potentially important regions for further dissection by molecular genetic techniques. This report demonstrates the advantages of combining multiple techniques in order to obtain a detailed description of cytogenetic changes in melanoma.
Volume
131
Issue
2
First Page
97
Last Page
103
ISSN
0165-4608
Published In/Presented At
Wiltshire, R. N., Dennis, T. R., Sondak, V. K., Meltzer, P. S., and Trent, J. M. (2001). Application of molecular cytogenetic techniques in a case study of human cutaneous metastatic melanoma. Cancer Genetics And Cytogenetics, 131(2), 97-103.
Disciplines
Medical Pathology | Pathology
PubMedID
11750048
Department(s)
Department of Pathology and Laboratory Medicine, Pathology Laboratory Medicine Faculty
Document Type
Article