Acridine orange-nucleic acid fluorescence. Its use in routine diagnostic muscle biopsies.
Publication/Presentation Date
10-1-1980
Abstract
Acridine orange-RNA fluorescence in diagnostic muscle biopsies is a sensitive, specific method to identify single fibers undergoing neurogenic atrophy. Fibers thus identified may be clearly distinguished from nonfluorescent type-specific atrophic fibers, as well as from atrophic fibers encountered in other myopathic conditions. Regenerating fibers, inflammatory cells, and mast cells stain prominently with acridine orange but area easily identified by their morphologic features. Acridine orange-RNA fluorescence is extremely useful in the evaluation of early neurogenic atrophy without fiber-type grouping and may be performed on paraffin-embedded sections, thus allowing retrospective studies. In addition, this technique may provide clues to the pathogenesis of neuromuscular disease that involves altered nucleic acid metabolism.
Volume
37
Issue
10
First Page
641
Last Page
644
ISSN
0003-9942
Published In/Presented At
Perl, D. P., & Little, B. W. (1980). Acridine orange-nucleic acid fluorescence. Its use in routine diagnostic muscle biopsies. Archives of neurology, 37(10), 641–644. https://doi.org/10.1001/archneur.1980.00500590065011
Disciplines
Medicine and Health Sciences
PubMedID
6158935
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article