Heterogeneity of neutral ribonuclease II and ribonuclease II-inhibitor complex from mouse skeletal muscle.
Publication/Presentation Date
9-28-1981
Abstract
Using either DEAE-cellulose chromatography or pH 4--6 isoelectric focusing, we have separated mouse skeletal muscle neutral RNAase II-inhibitor complex into two fractions (designated alph and beta). para-Hydroxymercuriphenyl-sulfonate-induced dissociation/inactivation of the inhibitor yields free RNAase II enzyme fractions with differing pH profiles, CM-cellulose chromatographic behavior and reactivity with the RNAase II inhibitor of human placenta. However, the free RNAase fractions react equally with purified inhibitor from skeletal muscle and are not separable by pH 8--9.5 isoelectric focusing. These data suggest that mouse skeletal muscle has two heterogeneous forms of RNAase II. Additionally, heterologous RNAase II inhibitors may be used as investigational tools when probing neutral RNAase II heterogeneity.
Volume
655
Issue
2
First Page
251
Last Page
255
ISSN
0006-3002
Published In/Presented At
Little, B. W., & Whittingham, L. S. (1981). Heterogeneity of neutral ribonuclease II and ribonuclease II-inhibitor complex from mouse skeletal muscle. Biochimica et biophysica acta, 655(2), 251–255. https://doi.org/10.1016/0005-2787(81)90015-0
Disciplines
Medicine and Health Sciences
PubMedID
6269627
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article