Lung tumor-associated antigens: thin layer immunoassay.
Publication/Presentation Date
1-1-1983
Abstract
Six hundred eighty-four rapid, sensitive thin layer immunoassays (TLI) were performed using an adapted visualization of condensation on plastic surface (VCS) method, for testing squamous cell and adenocarcinoma monospecific (TAA) and monoclonal antibody derived lung tumor-associated antigens (TAA epitopes) against small amounts of coded human test sera. The TAA epitopes selected for VCS-TLI were more specific, but both forms of TAA gave positive tests in 94% of lung cancer test sera of the appropriate histological types. The four antigens (adeno TAA and TAA epitope, squamous TAA and TAA epitope) did not react in VCS-TLI with coded sera from preselected normal individuals with known medical histories. With both TAA epitopes, 5% of non-lung cancer test sera were positive. There were indications that such tests may be useful in aiding pathological evaluations and in detecting precancerous conditions in patients with asbestosis. Cross-reacting (with other non-lung cancers) and specific peptide sequences of TAA were indicated in the comparative tests with the corresponding TAA epitopes, suggesting the tests for probing and analyzing portions of the antigens. Of immediate use is VCS-TLI for monitoring patients on specific active TAA immunotherapy.
Volume
6
Issue
1-2
First Page
185
Last Page
191
ISSN
0361-090X
Published In/Presented At
Hollinshead, A. C., Stewart, T. H., Hamilton, R. L., & DiAngelo, C. R. (1983). Lung tumor-associated antigens: thin layer immunoassay. Cancer detection and prevention, 6(1-2), 185–191.
Disciplines
Medicine and Health Sciences
PubMedID
6883379
Department(s)
Department of Pathology and Laboratory Medicine
Document Type
Article