Overcoming Biological Barriers with Ultrasound.
Publication/Presentation Date
1-1-2012
Abstract
Effect of ultrasound on the permeability of blood vessels and cell membranes to macromolecules and nanodroplets was investigated using mouse carotid arteries and tumor cells. Model macromolecular drug, FITC-dextran with molecular weight of 70,000 Da was used in experiments with carotid arteries. The effect of unfocused 1-MHz ultrasound and and perfluoro-15-crown-5-ether nanodroplets stabilized with the poly(ethylene oxide)-co-poly(D,L-lactide) block copolymer shells was studied. In cell culture experiments, ovarian carcinoma cells and Doxorubicin (DOX) loaded poly(ethylene oxide)-co-polycaprolactone nanodroplets were used. The data showed that the application of ultrasound resulted in permeabilization of all biological barriers tested. Under the action of ultrasound, not only FITC-dextran but also nanodroplets effectively penetrated through the arterial wall; the effect of continuous wave ultrasound was stronger than that of pulsed ultrasound. In cell culture experiments, ultrasound triggered DOX penetration into cell nuclei, presumably due to releasing the drug from the carrier. Detailed mechanisms of the observed effects require further study.
Volume
1481
First Page
381
Last Page
387
ISSN
0094-243X
Published In/Presented At
Thakkar, D., Gupta, R., Mohan, P., Monson, K., & Rapoport, N. (2012). Overcoming Biological Barriers with Ultrasound. AIP conference proceedings, 1481, 381–387. https://doi.org/10.1063/1.4757365
Disciplines
Medicine and Health Sciences | Oncology
PubMedID
24839333
Department(s)
Department of Radiation Oncology
Document Type
Article