Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila.
Publication/Presentation Date
7-15-2005
Abstract
In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNA-binding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.
Volume
19
Issue
14
First Page
1674
Last Page
1679
ISSN
0890-9369
Published In/Presented At
Jiang, F., Ye, X., Liu, X., Fincher, L., McKearin, D., & Liu, Q. (2005). Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila. Genes & development, 19(14), 1674–1679. https://doi.org/10.1101/gad.1334005
Disciplines
Diagnosis | Medicine and Health Sciences | Other Analytical, Diagnostic and Therapeutic Techniques and Equipment | Radiology
PubMedID
15985611
Department(s)
Department of Radiology and Diagnostic Medical Imaging
Document Type
Article