Amelioration of graft versus host disease by galectin-1.
Publication/Presentation Date
12-1-2003
Abstract
Graft versus host disease is a significant cause of morbidity and mortality following allogeneic hematopoietic stem cell transplantation. Galectin-1, a mammalian lectin that modulates T cell function and apoptosis, has been shown to be immunomodulatory in animal models of autoimmune disease. We investigated the efficacy of galectin-1 in a murine model of graft versus host disease and found that 68% of galectin-1-treated mice survived, compared to 3% of vehicle-treated mice. Galectin-1-treated animals also had reduced inflammatory infiltrates in tissues compared to animals treated with vehicle alone. Galectin-1 did not affect engraftment of donor hematopoietic cells. However, galectin-1-treated animals demonstrated increased cellularity in bone marrow and spleen with increased numbers of splenic B cells and CD4 T cells compared to those animals treated with vehicle alone. Galectin-1 treatment also significantly improved reconstitution of normal splenic architecture following transplant. Production of type I cytokines interleukin-2 (IL-2) and interferon-gamma was reduced in splenocytes derived from galectin-1-treated transplanted mice when compared to animals treated with vehicle alone, while production of the type II cytokines, IL-4 and IL-10, was similar between the two groups of animals. Although splenocytes from galectin-1-treated transplanted animals responded to both third party antigens and leukemic challenge, host alloreactivity was significantly reduced when compared to cells from vehicle-treated animals. These results demonstrate that galectin-1 therapy is capable of increasing survival and suppressing the graft versus host immune response without compromising engraftment or immune reconstitution following allogeneic hematopoietic stem cell transplant.
Volume
109
Issue
3
First Page
295
Last Page
307
ISSN
1521-6616
Published In/Presented At
Baum, L. G., Blackall, D. P., Arias-Magallano, S., Nanigian, D., Uh, S. Y., Browne, J. M., Hoffmann, D., Emmanouilides, C. E., Territo, M. C., & Baldwin, G. C. (2003). Amelioration of graft versus host disease by galectin-1. Clinical immunology (Orlando, Fla.), 109(3), 295–307. https://doi.org/10.1016/j.clim.2003.08.003
Disciplines
Diagnosis | Medicine and Health Sciences | Other Analytical, Diagnostic and Therapeutic Techniques and Equipment | Radiology
PubMedID
14697744
Department(s)
Department of Radiology and Diagnostic Medical Imaging
Document Type
Article