Establishment and characterization of a new human urinary bladder carcinoma cell line (PS-1).
Publication/Presentation Date
11-1-1978
Abstract
An epithelioid cell line (PS-1) has been established from a transitional cell cancer derived from human urinary bladder. Subcutaneous injection of the epithelioid cells into weanling athymic nude mice induced solid tumors histologically similar to the original tumor. A cell line was also established from a tumor induced in the athymic nude mouse (PS-1, T-1). Both cell lines exhibited essentially identical growth characteristics and formed a monolayer growth of epithelioid cells in culture. Electron microscopic studies confirmed epithelioid morphology. No fibroblastoid elements were observed. Chromosomal analysis revealed heteroploidy with persistent marker chromosomes; all cells contained a Y chromosome. The presence of tumor-specific antigen(s) in PS-1 cells was suggested by microcytotoxicity assays with peripheral allogeneic lymphocytes from other transitional cancer cell patients. Sera of urinary bladder cancer patients reacted with nuclear antigens of the established cells.
Volume
16
Issue
3
First Page
246
Last Page
252
ISSN
0021-0005
Published In/Presented At
Sanford, E. J., Geder, L., Dagen, J. E., Laychock, A. M., Ladda, R., & Rohner, T. J., Jr (1978). Establishment and characterization of a new human urinary bladder carcinoma cell line (PS-1). Investigative urology, 16(3), 246–252.
Disciplines
Diagnosis | Medicine and Health Sciences | Other Analytical, Diagnostic and Therapeutic Techniques and Equipment | Radiology
PubMedID
81814
Department(s)
Department of Radiology and Diagnostic Medical Imaging
Document Type
Article