Trans-synaptic EphB2-ephrin-B3 interaction regulates excitatory synapse density by inhibition of postsynaptic MAPK signaling.
Publication/Presentation Date
5-11-2010
Abstract
Nervous system function requires tight control over the number of synapses individual neurons receive, but the underlying cellular and molecular mechanisms that regulate synapse number remain obscure. Here we present evidence that a trans-synaptic interaction between EphB2 in the presynaptic compartment and ephrin-B3 in the postsynaptic compartment regulates synapse density and the formation of dendritic spines. Observations in cultured cortical neurons demonstrate that synapse density scales with ephrin-B3 expression level and is controlled by ephrin-B3-dependent competitive cell-cell interactions. RNA interference and biochemical experiments support the model that ephrin-B3 regulates synapse density by directly binding to Erk1/2 to inhibit postsynaptic Ras/mitogen-activated protein kinase signaling. Together these findings define a mechanism that contributes to synapse maturation and controls the number of excitatory synaptic inputs received by individual neurons.
Volume
107
Issue
19
First Page
8830
Last Page
8835
ISSN
1091-6490
Published In/Presented At
McClelland, A. C., Hruska, M., Coenen, A. J., Henkemeyer, M., & Dalva, M. B. (2010). Trans-synaptic EphB2-ephrin-B3 interaction regulates excitatory synapse density by inhibition of postsynaptic MAPK signaling. Proceedings of the National Academy of Sciences of the United States of America, 107(19), 8830–8835. https://doi.org/10.1073/pnas.0910644107
Disciplines
Diagnosis | Medicine and Health Sciences | Other Analytical, Diagnostic and Therapeutic Techniques and Equipment | Radiology
PubMedID
20410461
Department(s)
Department of Radiology and Diagnostic Medical Imaging
Document Type
Article