Induction of retinal progenitors and neurons from mammalian Müller glia under defined conditions.

Authors

Jack Jiagang Zhao
Hong Ouyang
Jing Luo
Sherrina Patel
Yuanchao Xue
John Quach
Nicole Sfeir
Meixia Zhang
Xiangdong Fu
Sheng Ding
Shaochen Chen
Kang Zhang

Publication/Presentation Date

4-25-2014

Abstract

Vision impairment caused by loss of retinal neurons affects millions of people worldwide, and currently, there is no effective treatment. Müller glia of mammalian retina may represent an under-recognized and potential source for regeneration of a wide range of retinal cell types, including retinal ganglion cells and photoreceptors. Here, we demonstrated that mouse Müller glia cells have the capacity to be reprogrammed into the retinal neuronal cell fate and are competent to give rise to photoreceptors under a defined culture condition. Inactivation of p53 released proliferation restriction of Müller glia and significantly enhanced the induction of retinal progenitor from Müller glia in culture. Moreover, following the ocular transplantation, the Müller glia-derived progenitors were differentiated toward the fates of photoreceptors and retinal ganglion cells. Together, these results demonstrate the feasibility of using Müller glia as a potential source for retinal repair and regeneration.

Volume

289

Issue

17

First Page

11945

Last Page

11951

ISSN

1083-351X

Published In/Presented At

Zhao, J. J., Ouyang, H., Luo, J., Patel, S., Xue, Y., Quach, J., Sfeir, N., Zhang, M., Fu, X., Ding, S., Chen, S., & Zhang, K. (2014). Induction of retinal progenitors and neurons from mammalian Müller glia under defined conditions. The Journal of biological chemistry, 289(17), 11945–11951. https://doi.org/10.1074/jbc.M113.532671

Disciplines

Medical Education | Medicine and Health Sciences

PubMedID

24523410

Department(s)

USF-LVHN SELECT Program, USF-LVHN SELECT Program Students

Document Type

Article