USF-LVHN SELECT
Single-cell analyses identify circulating anti-tumor CD8 T cells and markers for their enrichment.
Publication/Presentation Date
4-5-2021
Abstract
The ability to monitor anti-tumor CD8+ T cell responses in the blood has tremendous therapeutic potential. Here, we used paired single-cell RNA and TCR sequencing to detect and characterize "tumor-matching" (TM) CD8+ T cells in the blood of mice with MC38 tumors or melanoma patients using the TCR as a molecular barcode. TM cells showed increased activation compared with nonmatching T cells in blood and were less exhausted than matching cells in tumors. Importantly, PD-1, which has been used to identify putative circulating anti-tumor CD8+ T cells, showed poor sensitivity for identifying TM cells. By leveraging the transcriptome, we identified candidate cell surface markers for TM cells in mice and patients and validated NKG2D, CD39, and CX3CR1 in mice. These data show that the TCR can be used to identify tumor-relevant cells for characterization, reveal unique transcriptional properties of TM cells, and develop marker panels for tracking and analysis of these cells.
Volume
218
Issue
4
ISSN
1540-9538
Published In/Presented At
Pauken, K. E., Shahid, O., Lagattuta, K. A., Mahuron, K. M., Luber, J. M., Lowe, M. M., Huang, L., Delaney, C., Long, J. M., Fung, M. E., Newcomer, K., Tsai, K. K., Chow, M., Guinn, S., Kuchroo, J. R., Burke, K. P., Schenkel, J. M., Rosenblum, M. D., Daud, A. I., Sharpe, A. H., … Singer, M. (2021). Single-cell analyses identify circulating anti-tumor CD8 T cells and markers for their enrichment. The Journal of experimental medicine, 218(4), e20200920. https://doi.org/10.1084/jem.20200920
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
33651880
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article