USF-LVHN SELECT
A Tet-Inducible CRISPR Platform for High-Fidelity Editing of Human Pluripotent Stem Cells.
Publication/Presentation Date
12-14-2022
Abstract
Pluripotent stem cells (PSCs) offer an exciting resource for probing human biology; however, gene-editing efficiency remains relatively low in many cell types, including stem cells. Gene-editing using the CRISPR-Cas9 system offers an attractive solution that improves upon previous gene-editing approaches; however, like other technologies, off-target mutagenesis remains a concern. High-fidelity Cas9 variants greatly reduce off-target mutagenesis and offer a solution to this problem. To evaluate their utility as part of a cell-based gene-editing platform, human PSC lines were generated with a high-fidelity (HF) tetracycline-inducible engineered
Volume
13
Issue
12
ISSN
2073-4425
Published In/Presented At
Jurlina, S. L., Jones, M. K., Agarwal, D., De La Toba, D. V., Kambli, N., Su, F., Martin, H. M., Anderson, R., Wong, R. M., Seid, J., Attaluri, S. V., Chow, M., & Wahlin, K. J. (2022). A Tet-Inducible CRISPR Platform for High-Fidelity Editing of Human Pluripotent Stem Cells. Genes, 13(12), 2363. https://doi.org/10.3390/genes13122363
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
36553630
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article