USF-LVHN SELECT
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus.
Publication/Presentation Date
7-27-2021
Abstract
The use of an authentic RNA template is critical to advance the fundamental knowledge of viral RNA synthesis that can guide both mechanistic discovery and assay development in virology. The RNA template of nonsegmented negative-sense (NNS) RNA viruses, such as the respiratory syncytial virus (RSV), is not an RNA molecule alone but rather a nucleoprotein (N) encapsidated ribonucleoprotein complex. Despite the importance of the authentic RNA template, the generation and assembly of such a ribonucleoprotein complex remain sophisticated and require in-depth elucidation. The main challenge is that the overexpressed RSV N binds non-specifically to cellular RNAs to form random nucleocapsid-like particles (NCLPs). Here, we established a protocol to obtain RNA-free N (N
Issue
173
ISSN
1940-087X
Published In/Presented At
Gao, Y., Ogilvie, C., Raghavan, A., Von Hoffmann, C., & Liang, B. (2021). Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus. Journal of visualized experiments : JoVE, (173), 10.3791/62010. https://doi.org/10.3791/62010
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
34398139
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article