USF-LVHN SELECT
Structural and functional characterization of G protein-coupled receptors with deep mutational scanning.
Publication/Presentation Date
10-21-2020
Abstract
The >800 human G protein-coupled receptors (GPCRs) are responsible for transducing diverse chemical stimuli to alter cell state- and are the largest class of drug targets. Their myriad structural conformations and various modes of signaling make it challenging to understand their structure and function. Here, we developed a platform to characterize large libraries of GPCR variants in human cell lines with a barcoded transcriptional reporter of G protein signal transduction. We tested 7800 of 7828 possible single amino acid substitutions to the beta-2 adrenergic receptor (β
Volume
9
ISSN
2050-084X
Published In/Presented At
Jones, E. M., Lubock, N. B., Venkatakrishnan, A. J., Wang, J., Tseng, A. M., Paggi, J. M., Latorraca, N. R., Cancilla, D., Satyadi, M., Davis, J. E., Babu, M. M., Dror, R. O., & Kosuri, S. (2020). Structural and functional characterization of G protein-coupled receptors with deep mutational scanning. eLife, 9, e54895. https://doi.org/10.7554/eLife.54895
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
33084570
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article