Modulation of cell adhesion and migration by the histone methyltransferase subunit mDpy-30 and its interacting proteins.

Authors

Bin Xia
Alexandra Joubert
Benjamin Groves
Kevin Vo
Davin Ashraf
Derek Djavaherian
Jason Awe
Ying Xiong
Jacqueline Cherfils
Dzwokai Ma

Publication/Presentation Date

7-23-2010

Abstract

We have previously shown that a subset of mDpy-30, an accessory subunit of the nuclear histone H3 lysine 4 methyltransferase (H3K4MT) complex, also localizes at the trans-Golgi network (TGN), where its recruitment is mediated by the TGN-localized ARF guanine nucleotide exchange factor (ArfGEF) BIG1. Depletion of mDpy-30 inhibits the endosome-to-TGN transport of internalized CIMPR receptors and concurrently promotes their accumulation at the cell protrusion. These observations suggest mDpy-30 may play a novel role at the crossroads of endosomal trafficking, nuclear transcription and adhesion/migration. Here we provide novel mechanistic and functional insight into this association. First, we demonstrate a direct interaction between mDpy-30 and BIG1 and locate the binding region in the N-terminus of BIG1. Second, we provide evidence that the depletion or overexpression of mDpy-30 enhances or inhibits cellular adhesion/migration of glioma cells in vitro, respectively. A similar increase in cell adhesion/migration is observed in cells with reduced levels of BIG1 or other H3K4MT subunits. Third, knockdown of mDpy-30, BIG1, or the RbBP5 H3K4MT subunit increases the targeting of beta1 integrin to cell protrusions, and suppression of H3K4MT activity by depleting mDpy-30 or RbBP5 leads to increased protein and mRNA levels of beta1 integrin. Moreover, stimulation of cell adhesion/migration via mDpy-30 knockdown is abolished after treating cells with a function-blocking antibody to beta1 integrin. Taken together, these data indicate that mDpy-30 and its interacting proteins function as a novel class of cellular adhesion/migration modulators partially by affecting the subcellular distribution of endosomal compartments as well as the expression of key adhesion/migration proteins such as beta1 integrin.

Volume

5

Issue

7

First Page

11771

Last Page

11771

ISSN

1932-6203

Published In/Presented At

Xia, B., Joubert, A., Groves, B., Vo, K., Ashraf, D., Djavaherian, D., Awe, J., Xiong, Y., Cherfils, J., & Ma, D. (2010). Modulation of cell adhesion and migration by the histone methyltransferase subunit mDpy-30 and its interacting proteins. PloS one, 5(7), e11771. https://doi.org/10.1371/journal.pone.0011771

Disciplines

Medical Education | Medicine and Health Sciences

PubMedID

20668708

Department(s)

USF-LVHN SELECT Program, USF-LVHN SELECT Program Students

Document Type

Article