USF-LVHN SELECT
A genome-wide investigation of microRNA expression identifies biologically-meaningful microRNAs that distinguish between high-risk and low-risk intraductal papillary mucinous neoplasms of the pancreas.
Publication/Presentation Date
1-1-2015
Abstract
BACKGROUND: Intraductal papillary mucinous neoplasms (IPMNs) are pancreatic ductal adenocarcinoma (PDAC) precursors. Differentiating between high-risk IPMNs that warrant surgical resection and low-risk IPMNs that can be monitored is a significant clinical problem, and we sought to discover a panel of mi(cro)RNAs that accurately classify IPMN risk status.
METHODOLOGY/PRINCIPAL FINDINGS: In a discovery phase, genome-wide miRNA expression profiling was performed on 28 surgically-resected, pathologically-confirmed IPMNs (19 high-risk, 9 low-risk) using Taqman MicroRNA Arrays. A validation phase was performed in 21 independent IPMNs (13 high-risk, 8 low-risk). We also explored associations between miRNA expression level and various clinical and pathological factors and examined genes and pathways regulated by the identified miRNAs by integrating data from bioinformatic analyses and microarray analysis of miRNA gene targets. Six miRNAs (miR-100, miR-99b, miR-99a, miR-342-3p, miR-126, miR-130a) were down-regulated in high-risk versus low-risk IPMNs and distinguished between groups (P
CONCLUSIONS: This pilot study highlights miRNAs that may aid in preoperative risk stratification of IPMNs and provides novel insights into miRNA-mediated progression to pancreatic malignancy. The miRNAs identified here and in other recent investigations warrant evaluation in biofluids in a well-powered prospective cohort of individuals newly-diagnosed with IPMNs and other pancreatic cysts and those at increased genetic risk for these lesions.
Volume
10
Issue
1
First Page
0116869
Last Page
0116869
ISSN
1932-6203
Published In/Presented At
Permuth-Wey, J., Chen, Y. A., Fisher, K., McCarthy, S., Qu, X., Lloyd, M. C., Kasprzak, A., Fournier, M., Williams, V. L., Ghia, K. M., Yoder, S. J., Hall, L., Georgeades, C., Olaoye, F., Husain, K., Springett, G. M., Chen, D. T., Yeatman, T., Centeno, B. A., Klapman, J., … Malafa, M. (2015). A genome-wide investigation of microRNA expression identifies biologically-meaningful microRNAs that distinguish between high-risk and low-risk intraductal papillary mucinous neoplasms of the pancreas. PloS one, 10(1), e0116869. https://doi.org/10.1371/journal.pone.0116869
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
25607660
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article