USF-LVHN SELECT
VIP modulates the ALX/FPR2 receptor axis toward inflammation resolution in a mouse model of bacterial keratitis.
Publication/Presentation Date
2-1-2019
Abstract
Vasoactive intestinal peptide (VIP) has been shown to regulate corneal inflammation. Formyl peptide receptor 2 (FPR2) is a transmembrane protein belonging to the GPCR family. Ligands include pro-resolving lipids, lipoxin A4 (LXA4) and resolvin D1 (RvD1). The current study focuses on the effect of VIP regarding the FPR2 receptor axis in improving disease outcome in a mouse model of bacterial keratitis. Infection was induced in C57BL/6 (B6) mice using P. aeruginosa (PA) ATCC 19660. Mice received topical treatment (VIP or PBS) 3× daily after infection. Mean clinical scores, bacterial plate counts, Griess and myeloperoxidase (MPO) assays indicate that topical VIP effectively abrogates the disease response. Findings also reveal that VIP influences FPR2 pathway activation independent of archetypal VIP receptors. Exploring the immunoresolving role of FPR2, its ligand RvD1 and related enzymes (5-LOX, 12/15-LOX), our results suggest a mechanism by which VIP treatment influences the disease response in bacterial keratitis, which could offer a therapeutic point of intervention for enhancing this pro-resolving circuit.
Volume
140
First Page
18
Last Page
25
ISSN
1098-8823
Published In/Presented At
Carion, T. W., Kracht, D., Strand, E., David, E., McWhirter, C., Ebrahim, A. S., & Berger, E. A. (2019). VIP modulates the ALX/FPR2 receptor axis toward inflammation resolution in a mouse model of bacterial keratitis. Prostaglandins & other lipid mediators, 140, 18–25. https://doi.org/10.1016/j.prostaglandins.2018.12.001
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
30529189
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article