USF-LVHN SELECT
Radiation induces iatrogenic immunosuppression by indirectly affecting hematopoiesis in bone marrow.
Publication/Presentation Date
5-12-2020
Abstract
The immune system plays a vital role in cancer therapy, especially with the advent of immunotherapy. Radiation therapy induces iatrogenic immunosuppression referred to as radiation-induced lymphopenia (RIL). RIL correlates with significant decreases in the overall survival of cancer patients. Although the etiology and severity of lymphopenia are known, the mechanism(s) of RIL are largely unknown. We found that irradiation not only had direct effects on circulating lymphocytes but also had indirect effects on the spleen, thymus, and bone marrow. We found that irradiated cells traffic to the bone marrow and bring about the reduction of hematopoietic stem cells (HSC) and progenitor cells. Using mass cytometry analysis (CyTOF) of the bone marrow, we found reduced expression of CD11a, which is required for T cell proliferation and maturation. RNA Sequencing and gene set enrichment analysis of the bone marrow cells following irradiation showed down-regulation of genes involved in hematopoiesis. Identification of CD11a and hematopoietic genes involved in iatrogenic immune suppression can help identify mechanisms of RIL.
Volume
11
Issue
19
First Page
1681
Last Page
1690
ISSN
1949-2553
Published In/Presented At
Kapoor, V., Collins, A., Griffith, K., Ghosh, S., Wong, N., Wang, X., Challen, G. A., Krambs, J., Link, D., Hallahan, D. E., & Thotala, D. (2020). Radiation induces iatrogenic immunosuppression by indirectly affecting hematopoiesis in bone marrow. Oncotarget, 11(19), 1681–1690. https://doi.org/10.18632/oncotarget.27564
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
32477458
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article