USF-LVHN SELECT
Alda-1 attenuates hyperoxia-induced mitochondrial dysfunction in lung vascular endothelial cells.
Publication/Presentation Date
6-17-2019
Abstract
Acute lung injury (ALI) is a major cause of morbidity and mortality worldwide, especially in aged populations. Mitochondrial damage is one of the key features of ALI. Hyperoxia-induced lung injury model in mice has been widely used for ALI study because it features many ALI phenotypes including, but not limited to, mitochondrial and vascular endothelial cell damage. Recently, accumulating evidence has shown that mitochondrial aldehyde dehydrogenase 2 (ALDH2) has a protective effect against oxidative stress mediated cell damage in epithelial cells. However, it is not known whether ALDH2 protects against oxidative stress in vascular endothelial cells. In this current study, we attempted to find the capacity of Alda-1 [(N-(1,3benzodioxol-5-ylmethyl)-2,6- dichloro-benzamide), an ALDH2 activator] to protect against oxidative stress in human microvascular endothelial cells (HMVEC). HMVEC pretreated with Alda-1 prior to hyperoxic exposure vs non-treated controls showed i) lower 4-hydroxynonenal (4-HNE) levels, ii) significantly decreased expressions of Bax and Cytochrome C, iii) partially restored activity and expression of ALDH2 and iv) significantly improved mitochondrial membrane potential. These results suggest that ALDH2 protein in lung vascular endothelial cells is a promising therapeutic target for the treatment of ALI and that Alda-1 is a potential treatment option.
Volume
11
Issue
12
First Page
3909
Last Page
3918
ISSN
1945-4589
Published In/Presented At
Patil, S. S., Hernández-Cuervo, H., Fukumoto, J., Narala, V. R., Saji, S., Borra, M., Alleyn, M., Lin, M., Soundararajan, R., Lockey, R., Kolliputi, N., & Galam, L. (2019). Alda-1 attenuates hyperoxia-induced mitochondrial dysfunction in lung vascular endothelial cells. Aging, 11(12), 3909–3918. https://doi.org/10.18632/aging.102012
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
31209184
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article