A Microdevice Platform Recapitulating Hypoxic Tumor Microenvironments.

Authors

Yuta Ando
Hoang P Ta
Daniel P Yen
Sang-Sin Lee
Sneha Raola
Keyue Shen

Publication/Presentation Date

11-9-2017

Abstract

Hypoxia plays a central role in cancer progression and resistance to therapy. We have engineered a microdevice platform to recapitulate the intratumor oxygen gradients that drive the heterogeneous hypoxic landscapes in solid tumors. Our design features a "tumor section"-like culture by incorporating a cell layer between two diffusion barriers, where an oxygen gradient is established by cellular metabolism and physical constraints. We confirmed the oxygen gradient by numerical simulation and imaging-based oxygen sensor measurement. We also demonstrated spatially-resolved hypoxic signaling in cancer cells through immunostaining, gene expression assay, and hypoxia-targeted drug treatment. Our platform can accurately generate and control oxygen gradients, eliminates complex microfluidic handling, allows for incorporation of additional tumor components, and is compatible with high-content imaging and high-throughput applications. It is well suited for understanding hypoxia-mediated mechanisms in cancer disease and other biological processes, and discovery of new therapeutics.

Volume

7

Issue

1

First Page

15233

Last Page

15233

ISSN

2045-2322

Published In/Presented At

Ando, Y., Ta, H. P., Yen, D. P., Lee, S. S., Raola, S., & Shen, K. (2017). A Microdevice Platform Recapitulating Hypoxic Tumor Microenvironments. Scientific reports, 7(1), 15233. https://doi.org/10.1038/s41598-017-15583-3

Disciplines

Medical Education | Medicine and Health Sciences

PubMedID

29123197

Department(s)

USF-LVHN SELECT Program, USF-LVHN SELECT Program Students

Document Type

Article