USF-LVHN SELECT
Short-Term Rapamycin Preconditioning Diminishes Therapeutic Efficacy of Human Adipose-Derived Stem Cells in a Murine Model of Multiple Sclerosis.
Publication/Presentation Date
9-30-2020
Abstract
Human adipose-derived stem cells (ASCs) show immense promise for treating inflammatory diseases, attributed primarily to their potent paracrine signaling. Previous investigations demonstrated that short-term Rapamycin preconditioning of bone marrow-derived stem cells (BMSCs) elevated secretion of prostaglandin E2, a pleiotropic molecule with therapeutic effects in the experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS), and enhanced immunosuppressive capacity in vitro. However, this has yet to be examined in ASCs. The present study examined the therapeutic potential of short-term Rapamycin-preconditioned ASCs in the EAE model. Animals were treated at peak disease with control ASCs (EAE-ASCs), Rapa-preconditioned ASCs (EAE-Rapa-ASCs), or vehicle control (EAE). Results show that EAE-ASCs improved clinical disease scores and elevated intact myelin compared to both EAE and EAE-Rapa-ASC animals. These results correlated with augmented CD4
Volume
9
Issue
10
ISSN
2073-4409
Published In/Presented At
Wise, R. M., Harrison, M. A. A., Sullivan, B. N., Al-Ghadban, S., Aleman, S. J., Vinluan, A. T., Monaco, E. R., Donato, U. M., Pursell, I. A., & Bunnell, B. A. (2020). Short-Term Rapamycin Preconditioning Diminishes Therapeutic Efficacy of Human Adipose-Derived Stem Cells in a Murine Model of Multiple Sclerosis. Cells, 9(10), 2218. https://doi.org/10.3390/cells9102218
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
33008073
Department(s)
USF-LVHN SELECT Program, USF-LVHN SELECT Program Students
Document Type
Article