USF-LVHN SELECT
Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women.
Publication/Presentation Date
5-19-2020
Abstract
Development of effective prevention and treatment strategies for pre-eclampsia is limited by the lack of accurate methods for identification of at-risk pregnancies. We performed small RNA sequencing (RNA-seq) of maternal serum extracellular RNAs (exRNAs) to discover and verify microRNAs (miRNAs) differentially expressed in patients who later developed pre-eclampsia. Sera collected from 73 pre-eclampsia cases and 139 controls between 17 and 28 weeks gestational age (GA), divided into separate discovery and verification cohorts, are analyzed by small RNA-seq. Discovery and verification of univariate and bivariate miRNA biomarkers reveal that bivariate biomarkers verify at a markedly higher rate than univariate biomarkers. The majority of verified biomarkers contain miR-155-5p, which has been reported to mediate the pre-eclampsia-associated repression of endothelial nitric oxide synthase (eNOS) by tumor necrosis factor alpha (TNF-α). Deconvolution analysis reveals that several verified miRNA biomarkers come from the placenta and are likely carried by placenta-specific extracellular vesicles.
Volume
1
Issue
2
ISSN
2666-3791
Published In/Presented At
Srinivasan, S., Treacy, R., Herrero, T., Olsen, R., Leonardo, T. R., Zhang, X., DeHoff, P., To, C., Poling, L. G., Fernando, A., Leon-Garcia, S., Knepper, K., Tran, V., Meads, M., Tasarz, J., Vuppala, A., Park, S., Laurent, C. D., Bui, T., Cheah, P. S., … Laurent, L. C. (2020). Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women. Cell reports. Medicine, 1(2), 100013. https://doi.org/10.1016/j.xcrm.2020.100013
Disciplines
Medical Education | Medicine and Health Sciences
PubMedID
32864636
Department(s)
USF-LVHN SELECT Program
Document Type
Article