Perichondrium directed cartilage formation in silk fibroin and chitosan blend scaffolds for tracheal transplantation.

Publication/Presentation Date

9-1-2011

Abstract

The purpose of this study was to investigate the potential of silk fibroin and chitosan blend (SFCS) biological scaffolds for the purpose of cartilage tissue engineering with applications in tracheal tissue reconstruction. The capability of these scaffolds as cell carrier systems for chondrocytes was determined in vitro and cartilage generation in vivo on engineered chondrocyte-scaffold constructs with and without a perichondrium wrapping was tested in an in vivo nude mouse model. SFCS scaffolds supported chondrocyte adhesion, proliferation, and differentiation, determined as features of the cells based on the spherical cell morphology, increased accumulation of glycosaminoglycans, and increased collagen type II deposition with time within the scaffold framework. Perichondrium wrapping significantly (P

Volume

7

Issue

9

First Page

3422

Last Page

3431

ISSN

1878-7568

Disciplines

Medicine and Health Sciences

PubMedID

21640205

Department(s)

Department of Surgery

Document Type

Article

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