Induction and Purification of C. difficile Phage Tail-Like Particles.
Publication/Presentation Date
1-1-2016
Abstract
Due to the inherent limitations of conventional antibiotics for the treatment of C. difficile infection (CDI), there is a growing interest in the development of alternative treatment strategies. Both bacteriophages and R-type bacteriocins, also known as phage tail-like particles (PTLPs), show promise as potential antibacterial alternatives for treating CDI. Similar to bacteriophages, but lacking a viral capsid and genome, PTLPs remain capable of killing target bacteria. Here we describe our experience in the induction and purification of C. difficile PTLPs. These methods have been optimized to allow production of concentrated, non-contractile, and non-aggregated samples for both sensitivity testing and structural electron microscopy studies.
Volume
1476
First Page
167
Last Page
175
ISSN
1940-6029
Published In/Presented At
Hegarty, J. P., Sangster, W., Ashley, R. E., Myers, R., Hafenstein, S., & Stewart, D. B., Sr (2016). Induction and Purification of C. difficile Phage Tail-Like Particles. Methods in molecular biology (Clifton, N.J.), 1476, 167–175. https://doi.org/10.1007/978-1-4939-6361-4_12
Disciplines
Medicine and Health Sciences
PubMedID
27507340
Department(s)
Department of Surgery
Document Type
Article