An in situ collagen-HA hydrogel system promotes survival and preserves the proangiogenic secretion of hiPSC-derived vascular smooth muscle cells.
Publication/Presentation Date
12-1-2020
Abstract
Human-induced pluripotent stem cell-derived vascular smooth muscle cells (hiPSC-VSMCs) with proangiogenic properties have huge therapeutic potential. While hiPSC-VSMCs have already been utilized for wound healing using a biomimetic collagen scaffold, an in situ forming hydrogel mimicking the native environment of skin offers the promise of hiPSC-VSMC mediated repair and regeneration. Herein, the impact of a collagen type-I-hyaluronic acid (HA) in situ hydrogel cross-linked using a polyethylene glycol-based cross-linker on hiPSC-VSMCs viability and proangiogenic paracrine secretion was investigated. Our study demonstrated increases in cell viability, maintenance of phenotype and proangiogenic growth factor secretion, and proangiogenic activity in response to the conditioned medium. The optimally cross-linked and functionalized collagen type-I/HA hydrogel system developed in this study shows promise as an in situ hiPSC-VSMC carrier system for wound regeneration.
Volume
117
Issue
12
First Page
3912
Last Page
3923
ISSN
1097-0290
Published In/Presented At
Dash, B. C., Duan, K., Xing, H., Kyriakides, T. R., & Hsia, H. C. (2020). An in situ collagen-HA hydrogel system promotes survival and preserves the proangiogenic secretion of hiPSC-derived vascular smooth muscle cells. Biotechnology and bioengineering, 117(12), 3912–3923. https://doi.org/10.1002/bit.27530
Disciplines
Medicine and Health Sciences
PubMedID
32770746
Department(s)
Department of Surgery, Fellows and Residents, Department of Surgery Residents
Document Type
Article