Injectable bioadhesive hydrogels with innate antibacterial properties.
Publication/Presentation Date
6-24-2014
Abstract
Surgical site infections cause significant postoperative morbidity and increased healthcare costs. Bioadhesives used to fill surgical voids and support wound healing are typically devoid of antibacterial activity. Here we report novel syringe-injectable bioadhesive hydrogels with inherent antibacterial properties prepared from mixing polydextran aldehyde and branched polyethylenimine. These adhesives kill both Gram-negative and Gram-positive bacteria, while sparing human erythrocytes. An optimal composition of 2.5 wt% oxidized dextran and 6.9 wt% polyethylenimine sets within seconds forming a mechanically rigid (~1,700 Pa) gel offering a maximum adhesive stress of ~2.8 kPa. A murine infection model showed that the adhesive is capable of killing Streptococcus pyogenes introduced subcutaneously at the bioadhesive's surface, with minimal inflammatory response. The adhesive was also effective in a cecal ligation and puncture model, preventing sepsis and significantly improving survival. These bioadhesives represent novel, inherently antibacterial materials for wound-filling applications.
Volume
5
First Page
4095
Last Page
4095
ISSN
2041-1723
Published In/Presented At
Giano, M. C., Ibrahim, Z., Medina, S. H., Sarhane, K. A., Christensen, J. M., Yamada, Y., Brandacher, G., & Schneider, J. P. (2014). Injectable bioadhesive hydrogels with innate antibacterial properties. Nature communications, 5, 4095. https://doi.org/10.1038/ncomms5095
Disciplines
Medicine and Health Sciences
PubMedID
24958189
Department(s)
Department of Surgery
Document Type
Article