Glial Cell Line-Derived Neurotrophic Factor and Chondroitinase Promote Axonal Regeneration in a Chronic Denervation Animal Model.
Publication/Presentation Date
10-1-2019
Abstract
Functional recovery following nerve injury declines when target re-innervation is delayed. Currently, no intervention exists to improve outcomes after prolonged denervation. We explored the neuroregenerative effects of glial cell line-derived neurotrophic factor (GDNF) and chondroitinase (CDN) in a chronic denervation animal model. A fibrin-based sustained delivery method for growth factors was optimized in vitro and in vivo, and then tested in our animal model. GDNF, CDN, and GDNF+CDN were injected into the denervated stump at the time of nerve repair. Histomorphometry and retrograde labeling were used to assess axonal regeneration. The mechanisms promoting such regeneration were explored with immunofluorescence. Five weeks after repair, the GDNF+CDN group had the highest number and maturity of axons. GDNF was noted to preferentially promote axonal maturity, whereas CDN predominantly increased the number of axons. GDNF favored motor neuron regeneration, and upregulated Ki67 in Schwann cells. CDN did not favor motor versus sensory regeneration and was noted to cleave inhibitory endoneurial proteoglycans. Early measures of nerve regeneration after delayed repair are improved by activating Schwann cells and breaking down the inhibitory proteoglycans in the distal nerve segment, suggesting a role for GDNF+CDN to be translated for human nerve repairs.
Volume
16
Issue
4
First Page
1283
Last Page
1295
ISSN
1878-7479
Published In/Presented At
Sarhane, K. A., Tuffaha, S. H., Ibrahim, Z., Cashman, C. R., Krick, K., Martin, R., Broyles, J. M., Cooney, D. S., Lee, W. P. A., Mi, R., Mao, H. Q., Höke, A., & Brandacher, G. (2019). Glial Cell Line-Derived Neurotrophic Factor and Chondroitinase Promote Axonal Regeneration in a Chronic Denervation Animal Model. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 16(4), 1283–1295. https://doi.org/10.1007/s13311-019-00745-0
Disciplines
Medicine and Health Sciences
PubMedID
31148054
Department(s)
Department of Surgery
Document Type
Article