Transcriptional heterogeneity in human diabetic foot wounds.
Publication/Presentation Date
2-18-2023
Abstract
Wound repair requires the coordination of multiple cell types including immune cells and tissue resident cells to coordinate healing and return of tissue function. Diabetic foot ulceration is a type of chronic wound that impacts over 4 million patients in the US and over 7 million worldwide (Edmonds et al., 2021). Yet, the cellular and molecular mechanisms that go awry in these wounds are not fully understood. Here, by profiling chronic foot ulcers from non-diabetic (NDFUs) and diabetic (DFUs) patients using single-cell RNA sequencing, we find that DFUs display transcription changes that implicate reduced keratinocyte differentiation, altered fibroblast function and lineages, and defects in macrophage metabolism, inflammation, and ECM production compared to NDFUs. Furthermore, analysis of cellular interactions reveals major alterations in several signaling pathways that are altered in DFUs. These data provide a view of the mechanisms by which diabetes alters healing of foot ulcers and may provide therapeutic avenues for DFU treatments.
Published In/Presented At
Sandoval-Schaefer, T., Phan, Q., Dash, B. C., Prassinos, A. J., Duan, K., Gazes, M. I., Vyce, S. D., Driskell, R., Hsia, H. C., & Horsley, V. (2023). Transcriptional heterogeneity in human diabetic foot wounds. bioRxiv : the preprint server for biology, 2023.02.16.528839. https://doi.org/10.1101/2023.02.16.528839
Disciplines
Medicine and Health Sciences
PubMedID
36824808
Department(s)
Department of Surgery Residents, Fellows and Residents
Document Type
Article