A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro.

Authors

Yong Mao
Nikita John
Nicole M Protzman
Desiree Long
Raja Sivalenka
Shamshad Azimi
Brandon Mirabile
Robert Pouliot
Anna Gosiewska
Robert J Hariri
Stephen A. Brigido MD, Lehigh Valley Health NetworkFollow

Publication/Presentation Date

7-24-2023

Abstract

Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity.

Volume

34

Issue

7

First Page

37

Last Page

37

ISSN

1573-4838

Published In/Presented At

Mao, Y., John, N., Protzman, N. M., Long, D., Sivalenka, R., Azimi, S., Mirabile, B., Pouliot, R., Gosiewska, A., Hariri, R. J., & Brigido, S. A. (2023). A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro. Journal of materials science. Materials in medicine, 34(7), 37. https://doi.org/10.1007/s10856-023-06740-4

Disciplines

Medicine and Health Sciences

PubMedID

37486403

Department(s)

Department of Surgery

Document Type

Article