A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro.
Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity.
Published In/Presented At
Mao, Y., John, N., Protzman, N. M., Long, D., Sivalenka, R., Azimi, S., Mirabile, B., Pouliot, R., Gosiewska, A., Hariri, R. J., & Brigido, S. A. (2023). A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro. Journal of materials science. Materials in medicine, 34(7), 37. https://doi.org/10.1007/s10856-023-06740-4
Medicine and Health Sciences
Department of Surgery